Human multiple organ-reactive monoclonal autoantibody recognizes growth hormone and a 35,000-molecular weight protein.
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چکیده
منابع مشابه
Multiple organ-reactive IgG antibody induced by an antiidiotypic antibody to a human monoclonal IgM autoantibody
MOR-h1 is a human multiple organ-reactive (MOR) monoclonal autoantibody (Ab1) that reacts with human growth hormone (hGH) and a 35 kD protein found in the anterior pituitary, thyroid, stomach, and pancreas. 4E6 is a mouse monoclonal anti-idiotypic antibody (Ab2) that reacts with the paratope of MOR-h1 and is ligand inhibitable. In the present study, we immunized a rabbit with 4E6 and purified a...
متن کاملAnti - idiotypic Antibodies against a Human Multiple Organ - reactive Autoantibody
We have recently isolated and characterized a human monoclonal autoantibody, MOR-hl (multiple organ-reactive human 1), that reacts with antigens in multiple organs and have shown that this antibody binds to human growth hormone and a 35,000-mol wt protein. In the present study we generated three monoclonal antiidiotypic antibodies (4E6, 3E5, and 3F6) against MOR-hl. These anti-idiotypic antibod...
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We prepared a heterohybrid cell line that secretes a human IgM monoclonal autoantibody that recognizes an antigen found on thrombin-activated or stored platelets. The surface expression of the epitope recognized by this autoantibody, 5E5, increases with time as platelets age in vitro, suggesting that it may represent a senescence or activation-specific antigen. 5E5 binds to the purified platele...
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The recent work of Fishman, Wilhelmi, and Russell (1,2) has made available a crystalline growth hormone isolated from the bovine anterior pituitary gland. It was of considerable interest to determine the sedimentation behavior of this protein in the ultracentrifuge and, at the same time, to obtain the molecular weight by this method. The amorphous preparation of Li, Evans, and Simpson (3) has n...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1984
ISSN: 0021-9738
DOI: 10.1172/jci111566